Engineering Periodic shRNA for Enhanced Silencing Efficacy
نویسندگان
چکیده
منابع مشابه
Periodic-shRNA molecules are capable of gene silencing, cytotoxicity and innate immune activation in cancer cells.
Large dsRNA molecules can cause potent cytotoxic and immunostimulatory effects through the activation of pattern recognition receptors; however, synthetic versions of these molecules are mostly limited to simple sequences like poly-I:C and poly-A:U. Here we show that large RNA molecules generated by rolling circle transcription fold into periodic-shRNA (p-shRNA) structures and cause potent cyto...
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ژورنال
عنوان ژورنال: Molecular Therapy
سال: 2016
ISSN: 1525-0016
DOI: 10.1038/mt.2016.69